BrdU 抗体 (FITC)
Quick Overview for BrdU 抗体 (FITC) (ABIN272340)
抗原
See all BrdU 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
-
-
免疫原
- Bromodeoxyuridine conjugated to Helix Pomatia Haemocyanin used to immunize BALB/c mice
-
亚型
- IgG1
-
-
anti-Bromodeoxyuridine (BrdU) antibody
BrdU FACS, IHC (p), ICC, IHC (fro) 宿主: 小鼠 Monoclonal Bu20a unconjugated
anti-Bromodeoxyuridine (BrdU) antibodyBrdU IHC (p), IF (cc), IF (p), IHC (fro), WB 宿主: 小鼠 Monoclonal A7 unconjugated
anti-Bromodeoxyuridine (BrdU) antibodyBrdU IHC (p), IF (cc), IF (p), IHC (fro), ELISA 宿主: 兔 Polyclonal unconjugated
anti-Bromodeoxyuridine (BrdU) antibodyBrdU IHC, FACS, IF, WB, ELISA, FM, DB 宿主: 小鼠 Monoclonal 29G6-E8 unconjugated
anti-Bromodeoxyuridine (BrdU) antibodyBrdU IHC, Neut, StM 宿主: 小鼠 Monoclonal BRD469 unconjugated
anti-Bromodeoxyuridine (BrdU) antibodyBrdU IHC (p), ICC, IF (cc), IF (p), IHC (fro), ELISA 宿主: 兔 Polyclonal unconjugated
anti-Bromodeoxyuridine (BrdU) antibodyBrdU 适用: 人, 小鼠, 大鼠 IHC, IF 宿主: 小鼠 Monoclonal 4B6C12 unconjugated
anti-Bromodeoxyuridine (BrdU) antibodyBrdU IHC, Neut, StM 宿主: 兔 Monoclonal BRD2888R unconjugated Recombinant Antibody
anti-Bromodeoxyuridine (BrdU) antibody (PE)BrdU ICFC 宿主: 小鼠 Monoclonal Bu20a PE
-
-
应用备注
-
Immunohistochemistry. Flow Cytometry (1-5 μg/10^6 cells). This antibody is also useful for detecting proliferating cells by Flow cytometry or Immunofluorescence staining. The FITC conjugate is particularly useful in these applications as a secondary antibody and not necessary for visualization. Also, a specific anti-FITC antibody conjugate can be used for signal amplification if desired.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
-
-
-
浓度
- 1.0 mg/mL
-
缓冲液
- PBS containing 0.08 % Sodium Azide as a preservative.
-
储存液
- Sodium azide
-
注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- -20 °C
-
储存方法
-
Store the antibody (in aliquots) at -20 °C. Avoid repeated freezing and thawing.
Shelf Life: One year from despatch. -
有效期
- 12 months
-
-
- BrdU (Bromodeoxyuridine (BrdU))
-
物质类
- Chemical
-
背景
- Bromodexyuridine (BrdU) is a thymidine analog which is selectively incorporated into the DNA of proliferating cells to provide a marker for the DNA being replicated. The number of proliferating cells can then be detected in cell lysates, tissue sections or suspensions using an antibody specific for the BrdU. Previous methods of detecting DNA included the use of [3H]-thymidine which would be incorporated into the DNA and could then the DNA could be quantified by autoradiography or scintillation counting. These methods are more difficult and require more cleanup due to the radioactive material. An immunohistochemical assay provides a much simpler and cleaner method for detecting DNA in cells.
抗原
-
